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KMID : 1024519960050050605
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Journal of the Environmental Sciences
1996 Volume.5 No. 5 p.605 ~ p.610
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Isolation and Partial Characterization of Bacteriophage from Oyster
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Kim Young-Hee
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Abstract
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The bacteriophage from the fresh oyster, Crassostrea Virginica which is specific to the marine bacterium was isolated and characterized. Among the foci different vibrio species and the five different serotypes of Vibrio parahaemolyticus host strains tested, only two strains of the parahaemolyticus possessing K17, K52 antigens were highly sensitive to the phage. The size of the isolated plaque was 0.4mm and the electron microscopic head size of the isolated phage was about 67 nm long and 83 nm wide. PFU/ml was 1.25 . The phase was sensitive to chloroform but resistant to acetone or methanol. The assay of the isolated phase nucleic acid was deoxyribonucleic acid. The restriction enzyme pattern showed 14 fragment from Hind III and 4 fragments from Eco R I. Two different antigenic groups showed-similar restriction enzyme patterns.
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KEYWORD
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Bacteriophage, Crassostrea Virginica, Vibro parahaemolyticus, plaque, PFU: plaque-forming units, deoxyribonucleic acid, Eco R I, Hind III
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